Acetylcholinesterase sensors based on gold electrodes modified with dendrimer and polyaniline: A comparative research

详细信息    查看全文

• 作者：Snejdarkova ; M. ; Svobodova ; L. ; Evtugyn ; G. ; Budnikov ; H. ; Karyakin ; A. ; Nikolelis ; D.P. ; Hianik ; T.
• 关键词：Dendrimers ; Polyaniline ; Cholinesterase ; Enzyme sensor
• 刊名：Analytica Chimica Acta
• 出版年：2004
• 出版时间：June 30, 2004
• 年：2004
• 卷：514
• 期：1
• 页码：79-88
• 全文大小：145 K

文摘

Potentiometric and amperometric enzyme sensors based on modified gold electrodes have been developed and compared in pesticide determination. PAMAM dendrimer (generation G4) stabilized with 1-hexadecanethiol was used for the immobilization of acetylcholinesterase from electric eel and choline oxidase from Alcaligenes species in the assembly of amperometric sensor. Polyaniline-doped with camphorsulfonic acid was used to obtain potentiometric response. Trichlorfon, carbofuran and eserine suppress the biosensor response due to their inhibitory effect. The detection limits of 0.003 and 200nmoll⁻¹ (trichlorfon), 0.04 and 6nmoll⁻¹ (carbofuran) and 0.1 and 700nmoll⁻¹ were obtained for amperometric and potentiometric sensors, respectively. The difference in the biosensor behavior and the high sensitivity of the dendrimer modified sensor to the inhibitors is due to the specific organization of protein layer at charged surface of the modifier macromolecules.