Protein-bound tyrosine oxidation, nitration and chlorination by-products assessed by ultraperformance liquid chromatography coupled to tandem mass spectrometry
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- 作者：Isabel Torres-Cuevasa ; Julia Kuligowskia ; Marí ; a Cá ; rcela ; Consuelo Chá ; fer-Pericá ; sa ; Miguel Asensib ; Rø ; nnaug Solbergc ; Elena Cubellsa ; d ; Antonio Nuñ ; eza ; d ; Ola Didrik Saugstadc ; Má ; ximo Ventoa ; d ; e ; Javier Escobara ; justo.escobar@uv.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：Protein oxidation ; Protein nitration ; Phenylalanine ; Tyrosine ; Liquid chromatography ; Mass spectrometry ; Biomarkers ; Plasma ; Tissue
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：24 March 2016
- 年：2016
- 卷：913
- 期：Complete
- 页码：104-110
- 全文大小：468 K
文摘
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A validated liquid chromatographic method to determine protein-bound tyrosine oxidation, nitration and chlorination by-products.
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Plasma, Liver and Brain samples of hypoxic and normoxic animals from a postnatal hypoxia newborn piglets model.
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Small sample volume and suitable sample pre-treatment that includes protein extraction and subsequent enzymatic hydrolysis.
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High-throughput of sample analysis and high selectivity for Phe, p-Tyr, o-Tyr, m-Tyr, 3Cl-Tyr, 3NO2-Tyr phenylalanine, p-tyrosine, ortho-tyrosine, meta-tyrosine, 3-nitrotyrosine and 3-chlorotyrosine.