Tracking and quantification of nitrifying bacteria in biofilm and mixed liquor of a partial nitrification MBBR pilot plant using fluorescence in situ hybridization
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- 作者：Tarik Abzazoua ; tabzazou@ub.edu" class="auth_mail" title="E-mail the corresponding author ; Rosa M. Araujoa ; raraujo@ub.edu" class="auth_mail" title="E-mail the corresponding author ; Marí ; a Ausetb ; maria.auset.vallejo@acciona.com" class="auth_mail" title="E-mail the corresponding author ; Humbert Salvadó ; c ; hsalvado@ub.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：MBBR-PP ; moving bead biofilm reactor pilot plant ; AOB ; ammonia-oxidizing bacteria ; NOB ; nitrite-oxidizing bacteria ; FISH ; fluorescence in situ hybridization ; DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; V/V ; volume/volume ; PN ; partial nitrification ; Anammox ; anaerobic ammonium oxidation ; HRT ; hydraulic retention time ; DO ; dissolved oxygen ; PBS ; phosphate buffered saline ; Cy3 ; cyanine 3 ; AOA ; ammonia-oxidizing archaea ; TSS ; total suspended solids ; WWTP ; wastewater treatment plan
- 刊名：Science of the Total Environment
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：541
- 期：Complete
- 页码：1115-1123
- 全文大小：876 K
文摘
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Partial nitrification process in a MBBR fed with ammonium-rich liquor was achieved.
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The operational key parameters were the HRT and temperature.
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DAPI and FISH were useful to monitoring microbial composition of MBBR pilot plant.
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The AOB were the dominant nitrifying bacteria, presenting 11.3% of total bacteria.
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A significant correlation (R = 0.68) between AOB and ammonia removal was found.