Effects of metal ions and cosolutes on G-quadruplex topology

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• 作者：Taiga Fujii^a ; Peter Podbev&scaron ; ek^b ; ^c ; Janez Plavec^b ; ^c ; ^d ; Naoki Sugimoto^a ; ^e ; ^{sugimoto@konan-u.ac.jp}
• 关键词：PCR ; polymerase chain reaction ; dX ; 2&prime ; -deoxyxanthosine ; oxodG ; 8-oxo-2&prime ; -deoxyguanine ; CPG ; controlled pore glass ; ODN ; oligodeoxyribonucleotide ; NPE ; 2-(4-nitrophenyl)ethyl ; DBU ; 1 ; 8-diazabicyclo[5.4.0]undec-7-ene ; PAGE ; polyacrylamide gel electrophoresis ; UV ; ultraviolet ; PEG200 ; polyethylene glycol having an average molecular weight of 200 ; CD ; circular dichroism ; POT1 ; protection of telomeres 1 ; TPP1 ; POT1-interacting protein 1
• 刊名：Journal of Inorganic Biochemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：166
• 期：Complete
• 页码：190-198
• 全文大小：1793 K
• 卷排序：166

文摘

Modified bases at designed positions of a G-quadruplex controlled the topology. Na+ stabilized an antiparallel G-quadruplex, whereas K+ destabilized this topology. The cosolute did not stabilized the antiparallel G-quadruplex with KCl. The cosolute did stabilize the G-quadruplexes with NaCl. How metal ions and cosolutes regulate G-quadruplex topologies was discussed.