Identification of LI-F type antibiotics and di-n-butyl phthalate produced by Paenibacillus polymyxa

详细信息    查看全文

• 作者：Yang Deng^a ; Zhaoxin Lu^a ; Fengxia Lu^a ; Chong Zhang^a ; Yu Wang^a ; Haizhen Zhao^a ; Xiaomei Bie ; ^a ; ^{bxm43@njau.edu.cn"" rel=""nofollow}
• 关键词：Paenibacillus polymyxa ; Identification ; Mass spectrometry ; LI-F antibiotics ; Di-n-butyl phthalate
• 刊名：Journal of Microbiological Methods
• 出版年：2011
• 出版时间：June 2011
• 年：2011
• 卷：85
• 期：3
• 页码：175-182
• 全文大小：1282 K

文摘

Paenibacillus polymyxa strain JSa-9, a soil isolate that displayed antibacterial and antifungal activities in vitro, had been found to produce two types of antimicrobial substances. The two compounds were extracted from the fermentation broth of JSa-9 using ethyl acetate and subsequently purified by high performance liquid chromatography. By means of liquid chromatography–mass spectrometry and tandem mass spectrometry analysis, one of two antagonistic compounds was determined as di-n-butyl phthalate. And another was characterized as a mixture of related peptides of molecular masses of 883, 897, 911, 947, and 961 Da, with the most likely structure of them determined to be a cyclic depsipeptide with an unusual 15-guanidino-3-hydroxypentadecanoic acid moiety bound to a free amino group. These peptides were therefore members of the LI-F group of cyclic depsipeptides.