Detection of Salmonella enterica serovar Dublin by polymerase chain reaction in multiplex format

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• 作者：Ligong Zhai ; Xiaohan Kong ; Zhaoxin Lu ; Fengxia Lv ; Chong Zhang ; Xiaomei Bie ; ^{bxm43@njau.edu.cn" class="auth_mail}
• 关键词：S. Dublin ; Multiplex PCR ; Comparative genomic method ; Detection
• 刊名：Journal of Microbiological Methods
• 出版年：May, 2014
• 年：2014
• 卷：100
• 期：Complete
• 页码：52-57
• 全文大小：586 K

文摘

S. Dublin has caused widespread concerns in cattle produce. Using a comparative genomic method, two specific targets like SeD_A1118 and SeD_A2283 for S. Dublin identification were firstly obtained. An efficient multiplex PCR for S. Dublin detection based on the two novel specific genes and invA was therefore developed.