Identification and expressional analysis of NLRC5 inflammasome gene in smolting Atlantic salmon (Salmo salar)

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• 作者：Juan Pablo Pontigo^a ; ^b ; ^{jppontigo@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Marí ; a José ; Agü ; ero^a ; Patricio Sá ; nchez^c ; Ricardo Oyarzú ; n^a ; ^d ; Carolina Vargas-Lagos^a ; ^e ; Jorge Mancilla^d ; ^f ; Hans Kossmann^g ; Francisco J. Morera^h ; Alejandro J. Yá ; ñ ; ez^c ; ⁱ ; ^{ayanez@uach.cl" class="auth_mail" title="E-mail the corresponding author} ; Luis Vargas-Chacoff^a ; ^j ; ^{luis.vargas@uach.cl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Innate immunity ; Inflammation ; Leucine-rich repeats ; NLR ; NOD-like receptors ; Nucleotide binding ; Pathogen
• 刊名：Fish & Shellfish Immunology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：58
• 期：Complete
• 页码：259-265
• 全文大小：3004 K

文摘

The NOD-like receptors (NLRs) were recently identified as an intracellular pathogen recognition receptor family in vertebrates. While the immune system participation of NLRs has been characterized and analyzed in various mammalian models, few studies have considered NLRs in teleost species. Therefore, this study analyzed the Atlantic salmon (Salmo salar) NLRC5. Structurally, Atlantic salmon NLRC5 presented leucine-rich repeat subfamily genes. Phylogenetically, NLRC5 was moderately conserved between S. salar and other species. Real-time quantitative PCR revealed NLRC5 expression in almost all analyzed organs, with greatest expressions in the head kidney, spleen, and hindgut. Furthermore, NLRC5 gene expression decreased during smolt stage. These data suggest that NLRC5 participates in the Atlantic salmon immune response and is regulated, at least partly, by the smoltification process, suggesting that there is a depression of immune system from parr at smolt stage. This is the first report on the NLRC5 gene in salmonid smolts.