Observation for redox state of human serum and aqueous humor albumin from patients with senile cataract

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• 作者：Tomoya Hayashi ; Seiichi Era ; Kenji Kawai ; Hajime Imai ; Koji Nakamura ; Eiji Onda ; Mutsumi Yoh
• 刊名：Pathophysiology
• 出版年：2000
• 出版时间：February 2000
• 年：2000
• 卷：6
• 期：4
• 页码：237-243
• 全文大小：140 K

文摘

Human serum albumin is known to be a mixture of mercaptalbumin (HMA, reduced form) and nonmercaptalbumin (HNA, oxidized form), i.e. a protein redox couple in serum. In order to examine the redox state of human albumin in aqueous humor together with serum, we have studied 51 senile cataract patients, including 16 men and 35 women with ages ranging between 54 and 96 years (mean±S.D. 73.9±8.51). We used a high-performance liquid chromatography combined with fluorescence detection with improved sensitivity. The patients had no detectable evidence of other eye diseases. Of considerable interest is the finding that there was an extremely small amount of HMA fraction (f(HMA)) ((HMA), 3.50 % ) as well as a markedly large amount of HNA fraction (f(HNA)) ((HNA-1), 84.5 % ; (HNA-2), 12.0 % ) in the aqueous humor from patients with senile cataract, compared to the corresponding serum values ((HMA), 66.4 % ; (HNA-1), 31.0 % ; (HNA-2), 2.62 % ). It is anticipated that significantly decreased (HMA) and increased (HNA) values in aqueous humor from patients with senile cataract may clearly reflect defective antioxidative defense systems which may lead to the formation of a cataract. To the best of our knowledge, this is the first study observing the redox state of human albumin in aqueous humor.