Microglia derived from the axotomized adult rat facial nucleus uptake glutamate and metabolize it to glutamine in vitro

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• 作者：Kazuyuki Nakajima^a ; ^{nakajima@t.soka.ac.jp} ; Tomoyuki Kanamatsu^a ; Maasa Koshimoto^a ; Shinichi Kohsaka^b
• 关键词：Microglia ; Axotomized facial nucleus ; Glutamate transporter ; Glutamine synthetase ; Nuclear magnetic resonance
• 刊名：Neurochemistry International
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：102
• 期：Complete
• 页码：1-12
• 全文大小：2153 K
• 卷排序：102

文摘

Axotomized rat facial nucleus-derived microglia were isolated and cultured in vitro. The microglia were sub-cultured non-invasively by a specific polymer-grafted dish. The microglia showed a specific uptake of 14C-Glu by a glutamate transporter, GLT-1. The microglia expressed glutamine synthetase (GS) protein and showed GS activity. The microglia were suggested to change the uptaken 13C-Glu to 13C-Gln by their GS.