Bacterial infection (BI)-INDEX: an improved and simplified rapid flow cytometric bacterial infection marker

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• 作者：Jari Nuutila ; P盲ivi Jalava-Karvinen ; Ulla Hohenthal ; Pirkko Kotilainen ; Tarja-Terttu Pelliniemi ; Jukka Nikoskelainen ; Esa-Matti Lilius
• 关键词：Flow cytometry ; Bacterial infection viral infection ; Differential diagnosis ; Cell surface receptors ; Neutrophil ; Monocyte ; B-lymphocyte
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：February, 2014
• 年：2014
• 卷：78
• 期：2
• 页码：116-126
• 全文大小：2393 K

文摘

The aim of this study was to develop a rapid and simple flow cytometric bacterial infection marker. In this prospective comparative study, quantitative flow cytometric analysis of CD10, CD35, CD66b, CD282, and MHC Class I molecules on human neutrophils, monocytes, and B-lymphocytes from 141 hospitalized febrile patients with suspected infection and from 50 healthy controls was performed. We developed a flow cytometric marker of local and systemic bacterial infections, designated 鈥渂acterial infection (BI)-INDEX鈥? incorporating the quantitative analysis of CD10, CD35, MHCI, CD66b, and CD282 on neutrophils, monocytes, and B-lymphocytes, which displayed 90% sensitivity and 96% specificity in distinguishing between microbiologically confirmed bacterial (n = 31) and viral infections (n = 27) within a 1-h time-frame. We propose that our novel rapid BI-INDEX test will be useful in assisting physicians to ascertain whether antibiotic treatment is required, thus limiting unnecessary antimicrobial usage.