Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets

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• 作者：Yongdae Shin¹ ; Joel Berry² ; Nicole Pannucci³ ; Mikko P. Haataja² ; Jared E. Toettcher³ ; ^{toettcher@princeton.edu} ; Clifford P. Brangwynne¹ ; ⁴ ; ^{cbrangwy@princeton.edu}
• 关键词：liquid-liquid phase separation ; gelation ; aggregation ; optogenetics ; ribonucleoprotein bodies ; membraneless organelles ; intracellular condensates ; fluisomes ; intrinsically disordered protein ; RNA binding protein
• 刊名：Cell
• 出版年：2017
• 出版时间：12 January 2017
• 年：2017
• 卷：168
• 期：1-2
• 页码：159-171.e14
• 全文大小：9787 K
• 卷排序：168

文摘

OptoDroplets enable light-activatable control of intracellular phase transitions Rapid growth and fast inactivation lead to droplet assembly in subcellular regions Cells driven to deep supersaturation form solid-like gels Gels are initially reversible but undergo aging into irreversible aggregates