- 作者：Natalie M. Zahr ; Dirk Mayer ; Torsten Rohlfing ; Michael P. Hasak ; Oliver Hsu ; Shara Vinco ; Juan Orduna ; Richard Luong ; Edith V. Sullivan ; Adolf Pfefferbaum
- 关键词：Ethanol ; imaging ; liver ; neurochemicals ; rat ; recovery ; ventricular system
- 刊名：Biological Psychiatry
- 出版年：2010
- 出版时间：1 May 2010
- 年：2010
- 卷：67
- 期：9
- 页码：846-854
- 全文大小：932 K
Background
The binge-drinking model in rodents using intragastric injections of ethanol (EtOH) for 4 days results in argyrophilic corticolimbic tissue classically interpreted as indicating irreversible neuronal degeneration. However, recent findings suggest that acquired argyrophilia can also identify injured neurons that have the potential to recover. The current in vivo magnetic resonance (MR) imaging and spectroscopy study was conducted to test the hypothesis that binge EtOH exposure would injure but not cause the death of neurons as previously ascertained postmortem.Methods
After baseline MR scanning, 11 of 19 rats received a loading dose of 5 g/kg EtOH via oral gavage, then a maximum of 3 g/kg every 8 hours for 4 days, for a total average cumulative EtOH dose of 43 ± 1.2 g/kg and average blood alcohol levels of 258 ± 12 mg/dL. All animals were scanned after 4 days of gavage (post-gavage scan) with EtOH (EtOH group) or dextrose (control [Con] group) and again after 7 days of abstinence from EtOH (recovery scan).
Results
Tissue shrinkage at the post-gavage scan was reflected by significantly increased lateral ventricular volume in the EtOH group compared with the Con group. At the post-gavage scan, the EtOH group had lower dorsal hippocampal N-acetylaspartate and total creatine and higher choline-containing compounds than the Con group. At the recovery scan, neither ventricular volume nor metabolite levels differentiated the groups.
Conclusions
Rapid recovery of ventricular volume and metabolite levels with removal of the causative agent argues for transient rather than permanent effects of a single EtOH binge episode in rats.