The protonation state around Tyr_D/Tyr_D^• in photosystem II is reflected in its biphasic oxidation kinetics

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• 作者：Johannes Sj&ouml ; holm¹ ; Felix Ho ; ^{felix.ho@kemi.uu.se} ; Nigar Ahmadova ; Katharina Brinkert² ; Leif Hammarstr&ouml ; m ; Fikret Mamedov ; ^{fikret.mamedov@kemi.uu.se} ; Stenbj&ouml ; rn Styring
• 关键词：Chl ; chlorophyll ; DAD ; 2 ; 3 ; 5 ; 6-tetramethyl-p-phenylenediamine ; CW EPR ; continuous wave electron paramagnetic resonance ; DMSO ; dimethyl sulfoxide ; H/D ; hydrogen-deuterium exchange ; HEPES ; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ; KIE ; kinetic isotope effect ; MES ; 2-(N-morpholino)ethanesulfonic acid ; PCET ; proton coupled electron transfer ; PSII ; photosystem II ; Pheo ; pheophytin ; QA and QB ; primary and secondary plastoquinone acceptor in PSII ; S1 ; S2 ; redox states of the water oxidizing e
• 刊名：Biochimica et Biophysica Acta (BBA) - Bioenergetics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1858
• 期：2
• 页码：147-155
• 全文大小：1027 K
• 卷排序：1858

文摘

Biphasic oxidation of Tyrosine D in Photosystem II was found over wide pL range. The slow oxidation phase of Tyrosine D exhibited kinetic isotope effect of 2.4. Mechanism of oxidation is connected to different water positions close to Tyrosine D.