Biohydrogen production using native carbon monoxide converting anaerobic microbial consortium predominantly Petrobacter sp.

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• 作者：Kannan Pakshirajan ; Joyabrata Mal
• 关键词：Microbial CO conversion ; Petrobacter sp. ; Biohydrogen production ; Synthesis gas ; Statistically designed experiments
• 刊名：International Journal of Hydrogen Energy
• 出版年：13 December, 2013
• 年：2013
• 卷：38
• 期：36
• 页码：16020-16028
• 全文大小：1174 K

文摘

In this study, anaerobic mixed microbial consortium isolated from a local sewage treatment plant in Guwahati, India, was used to convert carbon monoxide (CO) to hydrogen. The consortium was initially grown in acetate containing medium and later acclimatized to utilize CO as the sole carbon source for hydrogen production. By 16S rDNA analysis, the consortium was identified to be predominantly Petrobacter sp. Statistically designed experiments were then applied to optimize the CO conversion and hydrogen production by the anaerobic mixed consortium. To evaluate the significant factors that influenced the biohydrogen production, Plackett-Burman screening design of experiments was applied, which revealed that temperature and Fe²⁺ influenced the most on hydrogen production with P values less than 0.05 each. The effect due to pH and Ni²⁺ was less with P values 0.120 and 0.132, respectively. Concentration of Fe²⁺ and Ni²⁺ in the medium was then subsequently optimized by using Central Composite Design (CCD) of experiments followed by response surface methodology (RSM) which yielded the optimum value of 213聽mg/L for Fe²⁺ and 2.2聽mg/L for Ni²⁺. At these optimum conditions, 60.8聽mol hydrogen production was achieved which was 8% higher than that observed from the screening experiment.