Investigation on the formation, conversion and bioactivity of a G-quadruplex structure in the PALB2 gene

详细信息    查看全文

• 作者：Fangyuan Li^a ; Jiang Zhou^a ; ^{zhoujiang@pku.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Ming Xu^b ; Gu Yuan^a ;
• 关键词：PALB2 gene ; G-quadruplex ; ESI-MS
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：83
• 期：Complete
• 页码：242-248
• 全文大小：1636 K

文摘

There is a putative G-quadruplex sequence (PGS), 5′-G₃ACAG₄TTG₂TG₄ TAG₄CAG₂TTG₃-3′, in the promoter region of the PALB2 gene. According to the importance of PALB2 in the process of homologous recombination and breast cancer, and the hypothesis that G-quadruplexes might play a role in transcriptional regulation, we evaluated the formation of G-quadruplex from this complex sequence, as well as its structural information and importance for PALB2 transcription. The G-rich sequence was suggested to form G-quadruplex structure in the NH₄OAc solution with 3NH₄⁺ ion by ESI-MS spectrometry, and it was testified in the KCl solution by CD experiments which showed the typical 290 nm band and a high melting temperature. We also classified the guanines in the sequence into four types and demonstrated their roles in the formation of the G-quadruplex structure. In addition, a luciferase assay proved that the formation of the G-quadruplex down-regulate the transcription of the gene. Therefore, our findings provided a foundation for the formation and bioactivity of the G-quadruplex in the promoter region of PALB2 gene and a novel insight for understanding PALB2 gene regulation.