Development and statistical assessment of a paper-based immunoassay for detection of tumor markers

详细信息    查看全文

• 作者：Thiago Mazzu-Nascimento^a ; ^b ; Giorgio Gianini Morbioli^a ; ^b ; ^c ; Luis Aparecido Milan^d ; Fabiana Cristina Donofrio^e ; Carlos Alberto Mestriner^f ; Emanuel Carrilho^a ; ^b ; ^{emanuel@iqsc.usp.br}
• 关键词：Tumor markers ; Cancer ; Paper-based ELISA ; Youden index (J) ; Receiver operating characteristic curve (ROC)
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：950
• 期：Complete
• 页码：156-161
• 全文大小：981 K
• 卷排序：950

文摘

A paper-based sandwich immunoassay protocol for detection of tumor markers. A statistical approach to define cut-off values and measuring test's sensitivity, specificity and accuracy. A simple way to create a gray zone, avoiding false positive and false negative outcomes.