The cell wall anchored β-fructosidases of Lactobacillus paracasei: Overproduction, purification, and gene expression control

详细信息    查看全文

• 作者：Petya Velikova^a ; Kaloyan Petrov^b ; Penka Petrova^a ; ^{pepipetrova@yahoo.com} ; ^{kaloian04@yahoo.com}
• 关键词：β-Fructosidase ; Lactobacillus paracasei ; Inulin ; Starch ; RT-PCR ; Gene expression
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：52
• 期：Complete
• 页码：53-62
• 全文大小：1074 K
• 卷排序：52

文摘

Two different cell wall anchored ß-fructosidases of L. paracasei were purified. Enhanced production of ß-fructosidases was achieved by pH maintenance at 5.5. Protein disturbances after the second Big3 domain lead to ß-fructosidases truncation. The simultaneous utilization of starch/inulin substrates was proved by RT-PCR. Amy1 and inu genes are under common expression control in L. paracasei.