Revised structures for the predominant O-polysaccharides expressed by Burkholderia pseudomallei and Burkholderia mallei

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• 作者：Christian Heiss ; Mary N. Burtnick ; Rosemary A. Roberts ; Ian Black ; Parastoo Azadi ; Paul J. Brett
• 关键词：Burkholderia pseudomallei ; Burkholderia mallei ; Burkholderia thailandensis ; Lipopolysaccharide ; O-polysaccharide ; Structure
• 刊名：Carbohydrate Research
• 出版年：2013
• 出版时间：15 November, 2013
• 年：2013
• 卷：381
• 期：Complete
• 页码：6-11
• 全文大小：1010 K

文摘

O-Polysaccharides (OPS) were isolated from purified Burkholderia pseudomallei and Burkholderia mallei lipopolysaccharides by mild-acid hydrolysis and gel-permeation chromatography. 1-D and 2-D ¹H and ¹³C NMR spectroscopy experiments revealed that the OPS antigens were unbranched heteropolymers with the following structures:

Collectively, our results demonstrate that the predominant OPS antigens expressed by B. pseudomallei and B. mallei isolates are structurally more complex than previously described and provide evidence that different capping residues are used by these closely related pathogens to terminate chain elongation. Additionally, they confirm that Burkholderia thailandensis and B. pseudomallei express OPS antigens that are essentially identical to one another.