Measurement of brown adipose tissue mass using a novel dual-echo magnetic resonance imaging approach: A validation study

详细信息    查看全文

• 作者：Milja Holstila ; Kirsi A. Virtanen ; Tove J. Gr?nroos ; Jukka Laine ; Virva Lepom?ki ; Jani Saunavaara ; Irina Lisinen ; Markku Komu ; Jarna C. Hannukainen ; Pirjo Nuutila ; Riitta Parkkola ; Ronald J.H. Borra
• 关键词：MRI ; magnetic resonance imaging ; BAT ; brown adipose tissue ; WAT ; white adipose tissue ; 18FDG-PET ; 18  ; F-Fluorodeoxyglucose positron emission tomography ; CT ; computed tomography ; FDG ; fluorodeoxyglucose ; SPIR ; spectral presaturation inversion recovery
• 刊名：Metabolism
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：62
• 期：8
• 页码：1189-1198
• 全文大小：2402 K

文摘

Objective

The aim of this study was to evaluate and validate magnetic resonance imaging (MRI) for the visualization and quantification of brown adipose tissue (BAT) in vivo in a rat model. We hypothesized that, based on differences in tissue water and lipid content, MRI could reliably differentiate between BAT and white adipose tissue (WAT) and could therefore be a possible alternative for ¹⁸ F-Fluorodeoxyglucose Positron Emission Tomography (¹⁸FDG-PET), the current gold standard for non-invasive BAT quantification.

Materials/Methods

Eleven rats were studied using both ¹⁸FDG-PET/CT and MRI (1.5 T). A dual echo (in-and-out-of-phase) sequence was used, both with and without spectral presaturation inversion recovery (SPIR) fat suppression (DUAL-SPIR) to visualize BAT, after which all BAT was surgically excised. The BAT volume measurements obtained via ¹⁸FDG-PET/CT and DUAL-SPIR MR were quantitatively compared with the histological findings. All study protocols were reviewed and approved by the local ethics committee.

Results

The BAT mass measurements that were obtained using DUAL-SPIR MR subtraction images correlated better with the histological findings (P = 0.017, R = 0.89) than did the measurements obtained using ¹⁸FDG-PET/CT (P = 0.78, R = 0.15), regardless of the BAT metabolic activation state. Additionally, the basic feasibility of the DUAL-SPIR method was demonstrated in three human pilot subjects.

Conclusions

This study demonstrates the potential for MRI to reliably detect and quantify BAT in vivo. MRI can provide information beyond that provided by ¹⁸FDG-PET imaging, and its ability to detect BAT is independent of its metabolic activation state. Additionally, MRI is a low-cost alternative that does not require radiation.