Characterizing Enterovirus 71 and Coxsackievirus A16 virus-like particles production in insect cells
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- 作者：Balaji Somasundarama ; 1 ; Cindy Changa ; 1 ; Yuan Y. Fanb ; Pei-Yin Limc ; Jane Cardosac ; Linda Luaa ; l.lua@uq.edu.au" class="auth_mail" title="E-mail the corresponding author
- 关键词：Enterovirus ; Virus-like particle ; Vaccine ; Insect cell ; Field-flow fractionation
- 刊名：Methods
- 出版年：2016
- 出版时间：15 February 2016
- 年：2016
- 卷：95
- 期：Complete
- 页码：38-45
- 全文大小：1707 K
文摘
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Expression at 21 °C enhanced the production of EV71 and CVA16 VLPs in Sf9 cells.
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CVA16 expression yield is adversely affected by serial passaging of virus.
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Higher VLP yield is obtained with EV71 compared to CVA16.
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VLP size distributions of EV71 and CVA16 VLPs are characterized by AF4-MALS.