Purification and assembling a fused capsid protein as an enterovirus 71 vaccine candidate from inclusion bodies to pentamer-based nanoparticles

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• 作者：Ling Xue^a ; ^c ; ¹ ; Jiangning Liu^b ; ¹ ; Qi Wang^a ; ^c ; Chun Zhang^a ; Longfu Xu^a ; ^c ; Jian Luo^a ; Jian Wang^d ; Chuan Qin^b ; ^{qinchuan@pumc.edu.cn} ; Yongdong Liu^a ; ^{ydliu@ipe.ac.cn} ; Zhiguo Su^a
• 关键词：Enterovirus 71 (EV71) ; Virus-like particles (VLPs) ; Assembling ; Fused antigen ; Inclusion bodies
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：117
• 期：part_PA
• 页码：139-146
• 全文大小：1568 K
• 卷排序：117

文摘

A preparation process for a novel EV71 vaccine candidate was developed. The yield for the fused antigen is about 60 mg/L culture. Fused antigen self-assembled as nanoparticles and elicit a high immune response.