Passive transfer of Plasmodium falciparum
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- 作者：Paola A. Martí ; nez ; Nubia Y ; ar ; Liliana P. Lesmes ; Martha Forero ; Oscar Pé ; rez-Leal ; Manuel Elkin Patarroyo ; José ; Manuel Lozano
- 关键词：Pseudopeptide ; Ig isotype ; Murine malarial infection ; In vivo neutralizing activity ; Anti-malarial vaccine
- 刊名：Peptides
- 出版年：2009
- 出版时间：February 2009
- 年：2009
- 卷：30
- 期：2
- 页码：330-342
- 全文大小：1715 K
文摘
We have developed monoclonal antibodies directed against the pseudopeptide ψ-130, derived from the highly conserved malarial antigen Plasmodium falciparum merozoite surface protein 2 (MSP-2), for obtaining novel molecular tools with potential applications in the control of malaria. Following isotype switching, these antibodies were tested for their ability to suppress blood-stage parasitemia through passive immunization in malaria-infected mice. Some proved totally effective in suppressing a lethal blood-stage challenge infection and others reduced malarial parasitemia. Protection against P. berghei malaria following Ig passive immunization can be associated with specific immunoglobulins induced by a site-directed designed MSP-2 reduced amide pseudopeptide.