Development of an affinity cryogel for one step purification of lysozyme from chicken egg white

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• 作者：Paula Chequer Gouveia Mó ; l^a ; Lizzy Ayra Alcâ ; ntara Verí ; ssimo^b ; Monique Renon Eller^c ; Valé ; ria Paula Rodrigues Minim^c ; Luis Antonio Minim^c ; ^{lminim@ufv.br}
• 关键词：Lysozyme ; Cryogel ; Affinity chromatography ; Purification
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：1044-1045
• 期：Complete
• 页码：17-23
• 全文大小：1190 K
• 卷排序：1044

文摘

Polyacrylamide cryogel was synthetized by radical cryocopolymerization technique. Cryogel was activated with Tris for affinity adsorption of lysozyme from egg white. HETP was determined as a function of viscosity and flow velocity of mobile phase. Adsorption isotherms were determined at two different temperatures. Purification of LYZ was studied at different flow velocities and dilution rate of EW.