Integrated work-flow for quantitative metabolome profiling of plants, Peucedani Radix as a case

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• 作者：Yuelin Song^a ; ¹ ; Qingqing Song^a ; ^b ; ¹ ; Yao Liu^a ; ^b ; Jun Li^a ; Jian-Bo Wan^c ; Yitao Wang^c ; Yong Jiang^d ; ^{yongjiang@bjmu.edu.cn} ; Pengfei Tu^a ; ^{pengfeitu@163.com}
• 关键词：Quantitative metabolome ; Integrated work-flow ; Offline two dimensional liquid chromatography ; Linear range ; Peucedani Radix ; Isomer
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：8 February 2017
• 年：2017
• 卷：953
• 期：Complete
• 页码：40-47
• 全文大小：782 K
• 卷排序：953

文摘

Novel three-step workflow was proposed for non-targeted metabolomics. Optimal collision energy was firstly utilized for the discrimination of isomers. Linear ranges were extended by suppressing the responses with inferior parameters. Authentic compound-free method was implemented for parameter optimization. Universal quantitation was achieved by a set of diluted herbal extract mixture.