Nanoparticles (NPs) are attractive carriers for vaccines. We have previously shown that a short
peptide (
Hp91) activates dendritic cells (DCs), which are critical for initiation of immune responses. In an effort to develop H
p91 as a vaccine adjuvant with NP carriers, we evaluated its activity when encapsulated in or conjugated to the surface of poly(d,l-lactic-co-glycolic) acid (PLGA) NPs. We found that H
p91, when encapsulated in or conjugated to the surface of PLGA-NPs, not only activates both human and mouse DCs, but is in fact more potent than free H
p91. H
p91 packaged within NPs was about fivefold more potent than the free
peptide, and H
p91 conjugated to the surface of NPs was 20-fold more potent than free H
p91. Because of their capacity to activate DCs, such NP-H
p91 systems are promising as delivery vehicles for subunit vaccines against infectious disease or cancer.
From the Clinical Editor
In this paper, nanoparticle-based dendritic cell activating vaccines are described and discussed. The authors report that the presented PLGA NP based vaccine constructs increase the potency of the studied vaccine by up to 20-fold, making them promising as delivery vehicles for subunit vaccines against infectious diseases or cancer.