Charges drive selection of specific antibodies by phage display

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• 作者：Helena Persson ; Jonas Persson ; Lena Danielsson ; Mats Ohlin
• 关键词：Amino acid ; Antibody fragment ; Charge ; Phage display ; Selection
• 刊名：Journal of Immunological Methods
• 出版年：2010
• 出版时间：28 February 2010
• 年：2010
• 卷：353
• 期：1-2
• 页码：24-30
• 全文大小：363 K

文摘

Phage display technology has emerged as a leading approach to select proteins with improved properties for many different types of applications. The selection typically selects not only for improved binding properties but also for other factors such as efficiency of protein production and folding in Escherichia coli, the host in which the proteins and the phage are produced. Furthermore, the selection methodology is likely to influence the character of retrieved variants. We have now defined the extent whereby the charge of the displayed proteins influence the selection process, resulting in an increased average positive charge among selected proteins in comparison to the proteins that are harbored in the library before selection. Implications of and possible routes to minimize this effect are discussed.