Development of a TaqMan assay for sensitive detection of all pestiviruses infecting cattle, including the emerging HoBi-like strains
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- 作者：Michele Losurdoa ; Viviana Maria ; Maria Stella Lucentea ; Maria Loredana Colaiannib ; Iolanda Padalinob ; Nicola Cavaliereb ; Canio Buonavogliaa ; b ; Nicola Decaroa ; nicola.decaro@uniba.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cattle ; Pestivirus ; HoBi-like viruses ; Real-time RT-PCR
- 刊名：Journal of Virological Methods
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：224
- 期：Complete
- 页码：77-82
- 全文大小：613 K
文摘
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A real-time RT-PCR assay was developed for detection of bovine pestiviruses.
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The assay was proven to be rapid, sensitive, and reproducible.
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The emerging HoBi-like strains were correctly detected by the new assay.