Meta analysis: Haplotype or single polymorphism analysis: Authors’ Reply to Comment on “Genetic polymorphisms in vasoactive genes and preeclampsia: A meta-analysis” [Eur. J. Obste

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• 作者：Borut Peterlin ; Igor Medica ; Andrej Kastrin
• 关键词：Genital prolapse ; Mesh ; Experimental model ; Infection
• 刊名：European Journal of Obstetrics and Gynecology and Reproductive Biology
• 出版年：2008
• 出版时间：February 2008
• 年：2008
• 卷：136
• 期：2
• 页码：260-261
• 全文大小：90 K

文摘

The objective was to develop an animal model using bacterial inoculation to evaluate tissue integration and tolerance to meshes used in genital prolapse surgery.

Study design

We placed three different meshes under the abdominal skin of 120 Wistar rats: a polypropylene monofilament non-coated mesh (Parietene^®), a polypropylene monofilament collagen-coated mesh (Ugytex^®) and a polyethylene terephthalate mesh (Mersuture^®). We performed bacterial inoculation just after implantation with 1 ml of 10⁷ colonies forming unit (CFU) of Staphylococcus epidermidis or Escherichia coli. Rats were sacrificed 7, 14, 60, and 90 days after intervention. We used polarised light microscopy to analyse the collagen deposition and organisation. We quantified the inflammation cells. Bacterial analysis and quantification of the explanted meshes were performed. The exact Fisher's test and Kruskal–Wallis test were used for statistics.

Results

We did not find any significant difference between inoculated or non-inoculated meshes in terms of collagen deposition. The scarring process seemed stable at day 90. Tissue integration was best with the polypropylene meshes, which allowed the development of a well-organised, mature connective tissue. Inflammatory reaction was higher in inoculated meshes, but only at day 7. At day 90, we found a high number of macrophages and multinuclear cells around all the meshes. There was no significant difference between prostheses that had been inoculated and those that had not with regard to positive bacterial culture. Quantification of bacterial colonies decreased with time.

Conclusion

In this animal model, we did not find any clinically related difference in infection and tissue integration between the meshes used in genital prolapse. Such experimental studies must be carried out whenever new prostheses become available before their use is validated in common practice.