Cisplatin electrochemical biosensor

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• 作者：Jitka Petrlova ; David Potesil ; Josef Zehnalek ; Bernd Sures ; Vojtech Adam ; Libuse Trnkova and Rene Kizek
• 关键词：Biosensor ; Metallothionein ; Cisplatin ; Platinum based cytostatic ; Adsorptive transfer stripping analysis ; Differential pulse voltammetry ; Human blood serum
• 刊名：Electrochimica Acta
• 出版年：2006
• 出版时间：15 July 2006
• 年：2006
• 卷：51
• 期：24
• 页码：5169-5173
• 全文大小：241 K

文摘

Platinum complexes play an important role in the chemotherapy of various tumour diseases. The aim of this paper was to investigate if a metallothionein (MT) modified hanging mercury drop electrode can be applied as a cisplatin electrochemical biosensor. The modification of the mercury electrode surface by MT and the determination of cisplatin were performed by adsorptive transfer stripping technique and differential pulse voltammetry. The detection limit (3 S/N) of cisplatin ([Pt^II(NH₃)₂Cl₂]⁰) calculated from the decrease of CdT peak was about 2.5 pmol in 5 μl (0.5 μM) at the interaction time of 400 s. Moreover, we tested the influence of human blood serum as a complex biological matrix on the way of determination of cisplatin. On the basis of the obtained results we estimated that we are able to determine tens of picomoles of cisplatin (5 μl drop) in the presence of human blood serum.