Pretreatment of cultured preadipocytes with arachidonic acid during the differentiation phase without a cAMP-elevating agent enhances fat storage after the maturation phase
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- 作者：Ferdous Khana ; Pinky Karim Syedaa ; Michael Nii N. Narteya ; Mohammad Shahidur Rahmana ; Mohammad Safiqul Islama ; Kohji Nishimurab ; Mitsuo Jisakaa ; Fumiaki Shonoc ; Kazushige Yokotaa ; yokotaka@life.shimane-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：AA ; arachidonic acid ; IBMX ; 3-isobutyl-1-methylxanthine ; COX ; cyclooxygenase ; PG ; prostaglandin ; PPAR ; peroxisome proliferator-activated receptor ; L-PGDS ; lipocalin-type PGD synthase ; 15d-PGJ2 ; 15-deoxy-Δ12 ; 14-PGJ2 ; DMEM-HEPES ; Dulbecco&rsquo ; s modified Eagle&rsquo ; s medium with HEPES ; FBS ; fetal bovine serum ; RT ; reverse transcriptase ; PCR ; polymerase chain reaction ; ELISA ; enzyme-linked immunosorbent assay ; GM ; growth medium ; DM ; differentiation medium ; MM ; maturation medium ; mPGES ; membrane-b
- 刊名：Prostaglandins and Other Lipid Mediators
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：16-27
- 全文大小：2345 K
文摘
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Effect of pretreatment of preadipocytes with AA on adipogenesis was investigated.
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AA stimulated adipogenesis program during differentiation phase without IBMX.
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MRE-269, a selective agonist of IP, was the most potent instead of AA.
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Exposure of preadipocytes to AA enhanced biosynthesis of pro-adipogenic PGI2.
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AA suppressed endogenous synthesis of anti-adipogenic prostanoids in preadipoccytes.