Catalytic properties and crystal structure of thermostable NAD(P)H-dependent carbonyl reductase from the hyperthermophilic archaeon Aeropyrum pernix K1
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- 作者：Yudai Fukudaa ; Haruhiko Sakurabab ; Tomohiro Arakia ; Toshihisa Ohshimac ; Kazunari Yonedaa ; kyoneda@agri.u-tokai.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：CR ; carbonyl reductase ; SDR ; short-chain dehydrogenase/reductase ; 9 ; 10-PQ ; 9 ; 10-phenanthrenequinone ; COBE ; ethyl 4-chloro-3-oxobutanoate ; (S)-CHBE ; ethyl (S)-4-chloro-3-hydroxybutanoate
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：91
- 期：Complete
- 页码：17-25
- 全文大小：2506 K
文摘
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An NAD(P)H-dependent carbonyl reductase (CR) from a hyperthermophilic archaeon was successfully isolated and characterized.
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10">The dimeric structure of the thermostable CR was determined using the molecular replacement method at a 2.09 Å resolution in the presence of NADPH.
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This is the first description of an archaeal CR and the structural characteristics of highly thermostable CR.