Membrane perturbing properties of natural phenolic and resorcinolic lipids

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• 作者：Maria Stasiuk ; Arkadiusz Kozubek
• 关键词：Antihemolysis ; Fluorescence polarization ; Hemolysis ; Permeability ; Phenolic lipids ; Resorcinolic lipids
• 刊名：FEBS Letters
• 出版年：2008
• 出版时间：29 October 2008
• 年：2008
• 卷：582
• 期：25-26
• 页码：3607-3613
• 全文大小：462 K

文摘

The effects induced by natural phenolic and resorcinolic lipids on membrane permeability were investigated. All of the compounds tested perturbed the phospholipid bilayer and stabilized erythrocytes against hypoosmotically induced hemolysis. Dipalmitoylphosphatidylcholine liposomes with two preincorporated fluorescent dyes (1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatrien p-toluenesulfonate (TMA-DPH) and N-(-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoetanolamine triethylammonium salt (NBD-PE)) were used to determine the effects of tested compounds on the core and surface of the bilayer. Resorcinolic lipids from rye and cardol increased the polarization of TMA-DPH fluorescence more than that of NBD-PE, but anacardic acid, methylocardol, and alkylphenol increased NBD-PE dye fluorescence.