Nicotinic receptor involvement in regulation of functions of mouse neutrophils from inflammatory site
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- 作者：Valentina G. Safronovaa ; safronova@icb.psn.ru" class="auth_mail" title="E-mail the corresponding author ; Catherine A. Vulfiusa ; Irina V. Shelukhinab ; Valentina N. Mal&rsquo ; tsevaa ; Alexey V. Berezhnova ; Eugeniya I. Fedotovaa ; Regina G. Miftahovaa ; c ; Elena V. Kryukovab ; Andrey A. Grinevicha ; Victor I. Tsetlinb
- 关键词：ACh ; acetylcholine ; BSA ; bovine serum albumin ; αBgt ; α-bungarotoxin ; [Ca2+]i ; intracellular free Ca2+ concentration ; αCTX ; α-cobratoxin ; fMLF ; N-formyl-l-methionyl-l-leucyl-l-phenylalanine ; HBSS ; Hanks&rsquo ; balanced salt solution ; IL-8 ; interleukin-8 ; LPS ; lipopolysaccharide ; MIP-2 ; macrophage inhibiting protein-2 ; MMP-9 ; matrix metallopeptidase-9 ; nAChR ; nicotinic acetylcholine receptor ; NF-kB ; nuclear factor-kB ; PKC ; protein kinase C ; PMSF ; phenylmethylsulfonyl fluoride ; PMN ; polymorphonucle
- 刊名：Immunobiology
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：221
- 期：7
- 页码：761-772
- 全文大小：2353 K
文摘
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Modifying action of nicotine and acetylcholine at low concentrations on N-formyl-MLF-induced ROS production and adhesion of neutrophils from inflammatory site of mice was demonstrated.
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Antagonists of nicotinic acetylcholine receptors (nAChRs) eliminated agonist effects. Nicotine induced Ca2+-spiking activity which was prevented by nAChR antagonist. RT-PCR analysis revealed the expression of mRNAs encoding α2, α3, α4, α5, α6, α7, α9, β2, β3, and β4 nAChR subunits.
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The data suggest involvement of nAChRs of α7 and possibly α3β2 or α6* subtypes in regulation of specific neutrophil functions.