IGPR-1 Is Required for Endothelial Cell-Cell Adhesion and Barrier Function

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• 作者：Yun Hwa Walter Wang¹ ; ^&dagger ; ; Rosana D Meyer¹ ; ^&dagger ; ; Philip A Bondzie¹ ; ^&dagger ; ; Yan Jiang² ; Ida Rahimi¹ ; Kobra Rezazadeh¹ ; Manisha Mehta¹ ; ⁴ ; Nora M.V. Laver³ ; Catherine E. Costello² ; Nader Rahimi¹ ; ^{nrahimi@bu.edu}
• 关键词：EC ; endothelial cell ; CAM ; cell adhesion molecule ; Ig-CAMs ; Ig-like cell adhesion proteins ; IGPR-1 ; Ig-containing and proline-rich receptor-1 ; PAE ; porcine aortic endothelial ; VE-cadherin ; vascular endothelial cadherin ; HUVEC ; human primary umbilical vein EC ; IGPR-1/PAE ; PAE cells ectopically expressing IGPR-1 ; TEER ; transendothelial electrical resistance ; GST ; glutathione S-transferase ; GST-E-IGPR-1 ; GST fusion extracellular domain of IGPR-1 ; LPS ; lipopolysaccharide ; ΔN-IGPR-1 ; extracellular do
• 刊名：Journal of Molecular Biology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：428
• 期：24
• 页码：5019-5033
• 全文大小：1849 K
• 卷排序：428

文摘

Molecular mechanisms of endothelial cell–cell adhesion and barrier function are not fully understood. IGPR-1 is a novel CAM expressed in ECs and is localized to endothelial adherens junctions. IGPR-1through trans-homophilic dimerization regulates endothelial cell–cell adhesion and barrier. Trans-homophilic dimerization of IGPR-1 stimulates the phosphorylation of Ser220, which is required for IGPR-1 to stimulate endothelial barrier function and angiogenesis.