The wheat MAP kinase phosphatase 1 alleviates salt stress and increases antioxidant activities in Arabidopsis

详细信息    查看全文

• 作者：Ikram Zaidi^a ; Chantal Ebel^a ; ^b ; Nibras Belgaroui^a ; Mouna Ghorbel^a ; Imè ; ne Amara^a ; Moez Hanin^a ; ^b ; ^{moez.hanin@cbs.rnrt.tn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：MAPK signaling ; TMKP1 ; Durum wheat ; Oxidative stress ; Salt stress tolerance
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：1 April 2016
• 年：2016
• 卷：193
• 期：Complete
• 页码：12-21
• 全文大小：1935 K

文摘

Mitogen-activated protein kinase phosphatases (MKPs) are important negative regulators in the MAPK signaling pathways, which play crucial roles in plant growth, development and stress responses. We have previously shown that the heterologous expression of a durum wheat MKP, TMKP1, results in increased tolerance to salt stress in yeast but its particular contribution in salt stress tolerance in plants was not investigated. Here, TMKP1 was overexpressed in Arabidopsis thaliana and physiological changes were assessed in transgenic plants exposed to stress conditions. Under salt stress and especially LiCl, the TMKP1 overexpressors displayed higher germination rates in comparison to wild type plants. The enhancement of salt stress tolerance was accompanied by increased antioxidant enzyme activities, namely superoxide dismutase, catalase and peroxydases. Such increases in antioxidant activities were concomitant with lower malondialdehyde, superoxide anion O₂⁻ and hydrogen peroxide levels in the TMKP1 transgenic seedlings. Moreover, we provide evidence that, in contrast to the Arabidopsis ortholog AtMKP1, TMKP1 acts as a positive regulator of salt stress tolerance via its ectopic expression in the Arabidopsis mkp1 mutant.