Simulation of outdoor pond cultures using indoor LED-lighted and temperature-controlled raceway ponds and Phenometrics photobioreactors

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• 作者：M. Huesemann^a ; ^{michael.huesemann@pnnl.gov} ; T. Dale^b ; A. Chavis^a ; B. Crowe^a ; S. Twary^b ; A. Barry^b ; D. Valentine^a ; R. Yoshida^b ; M. Wigmosta^c ; V. Cullinan^a
• 关键词：Phenometrics photobioreactors ; ePBR ; climate-simulation raceway ponds ; Chlorella sorokiniana ; Picochlorum soloecismus ; flow cytometry
• 刊名：Algal Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：21
• 期：Complete
• 页码：178-190
• 全文大小：1373 K
• 卷排序：21

文摘

Two innovative culturing systems, the LED-lighted and temperature-controlled 800 liter indoor raceways at Pacific Northwest National Laboratory (PNNL) and the Phenometrics environmental Photobioreactors™ (ePBRs) were evaluated in terms of their ability to accurately simulate the microalgae growth performance of outdoor cultures subjected to fluctuating sunlight and water temperature conditions. When repeating a 60-day outdoor pond culture experiment (batch and semi-continuous at two dilution rates) conducted in Arizona with the freshwater strain Chlorella sorokiniana DOE 1412 in these two indoor simulators, it was found that ash-free dry weight based biomass growth and productivity in the PNNL climate-simulation ponds was comparatively slightly higher (8–13%) but significantly lower (44%) in the ePBRs. The difference in biomass productivities between the indoor and outdoor ponds was not statistically significant. When the marine Picochlorum soloecismus was cultured in five replicate ePBRs at Los Alamos National Laboratory (LANL) and in duplicate indoor climate-simulation ponds at PNNL, using the same inoculum, medium, culture depth, and light and temperature scripts, the optical density based biomass productivity and the rate of increase in cell counts in the ePBRs was about 35% and 66%, respectively, lower compared than in the indoor ponds. Potential reasons for the divergence in growth performance in these pond simulators, relative to outdoor raceways, are discussed. In conclusion, the PNNL climate-simulation ponds provide reasonably reliable biomass productivity estimates for microalgae strains cultured in outdoor raceways under different climatic conditions.