Differential proteomics analysis to identify proteins and pathways associated with male sterility of soybean using iTRAQ-based strategy
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- 作者：Jiajia Li lijia6862@163.com" class="auth_mail" title="E-mail the corresponding author ; Xianlong Ding xlding2012@163.com" class="auth_mail" title="E-mail the corresponding author ; Shaohuai Han hmyh1202@163.com" class="auth_mail" title="E-mail the corresponding author ; Tingting He ht142857@163.com" class="auth_mail" title="E-mail the corresponding author ; Hao Zhang haozhang1223@163.com" class="auth_mail" title="E-mail the corresponding author ; Longshu Yang dashu6090@163.com" class="auth_mail" title="E-mail the corresponding author ; Shouping Yang ; spyung@126.com" class="auth_mail" title="E-mail the corresponding author ; Junyi Gai ; sri@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Soybean (Glycine max (L.) Merr.) ; Cytoplasmic male sterility (CMS) ; iTRAQ ; Proteomics ; Transcriptomics ; Conjoint analysis
- 刊名：Journal of Proteomics
- 出版年：2016
- 出版时间：14 April 2016
- 年：2016
- 卷：138
- 期：Complete
- 页码：72-82
- 全文大小：1316 K
文摘
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180 DAPs were identified between the CMS line NJCMS1A and its maintainer NJCMS1B in soybean using iTRAQ-based strategy.
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15 DAPs/DEGs with the same differential expression pattern were identified by the conjoint analysis of DAPs and DEGs.
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Bioinformatic analysis revealed that several key DAPs/DEGs might be associated with male sterility in soybean.
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The reliability of iTRAQ results were further confirmed by MRM test, qRT-PCR analysis and enzyme activity assay.
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