Differential induction of Pisum sativum defense signaling molecules in response to pea aphid infestation

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• 作者：Van Chung Mai^a ; ^b ; Kinga Drzewiecka^c ; Henryk Jele艅^d ; Dorota Naro偶na^e ; Renata Ruci艅ska-Sobkowiak^f ; Jacek K臋sy^g ; Jolanta Floryszak-Wieczorek^a ; Beata Gabry艣^h ; Iwona Morkunas^a ; ^{morkunas@jay.up.poznan.pl" class="auth_mail} ; ^{Iwona.Morkunas@gmail.com" class="auth_mail}
• 关键词：ACC synthase ; 1-aminocyclopropane-carboxylate synthase ; ACC oxidase ; 1-aminocyclopropane-carboxylate oxidase ; BA2H ; benzoic acid 2-hydroxylase ; cADPR ; cyclic ADP ribose ; ET ; ethylene ; FW ; fresh weight ; GACC ; 1-(纬-glutamyl)-ACC ; hpi ; hours post-infestation ; JA ; jasmonic acid ; JA/MeJA ; jasmonates ; LOX ; lipoxygenase ; MACC ; 1-(malonyl)-ACC ; MTA ; 5&prime ; -methylthioadenosine ; NO ; nitric oxide ; PAL ; phenylalanine ammonia-lyase ; RNS ; reactive nitrogen species ; ROS ; reactive oxygen species ; SA ; salicyl
• 刊名：Plant Science
• 出版年：May, 2014
• 年：2014
• 卷：221-222
• 期：Complete
• 页码：1-12
• 全文大小：2610 K

文摘

This study demonstrates the sequence of enhanced generation of signal molecules such as phytohormones, i.e. jasmonic acid (JA), ethylene (ET), salicylic acid (SA), and a relatively stable free radical, nitric oxide (NO), in response of Pisum sativum L. cv. Cysterski seedling leaves to the infestation of pea aphid Acyrthosiphon pisum (Harris) at a varied population size. In time from 0 to 96 h after A. pisum infestation these signal molecules accumulated transiently. Moreover, the convergence of these signaling pathways occurred. JA and its methyl derivative MeJA reached the first maximum of generation at 24th hour of infestation. An increase in ET and NO generation was observed at 48th hour of infestation. The increase in SA, JA/MeJA and ET concentrations in aphid-infested leaves occurred from the 72nd to 96th hour. In parallel, an increase was demonstrated for the activities of enzymes engaged in the biosynthesis of SA, such as phenylalanine ammonia-lyase (PAL) and benzoic acid 2-hydroxylase (BA2H). Additionally, a considerable post-infestation accumulation of transcripts for PAL was observed. An increase in the activity of lipoxygenase (LOX), an important enzyme in the biosynthesis of JA was noted. This complex signaling network may contribute to the coordinated regulation of gene expression leading to specific defence responses.