A bifunctional α-amylase/trypsin inhibitor from pigeonpea seeds: Purification, biochemical characterization and its bio-efficacy against Helicoverpa armigera
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- 作者：Prafull P. Gadgea ; Sandip K. Wagha ; Faiyaz K. Shaikha ; Rajesh D. Takb ; Manohar V. Padula ; manoharpadul@yahoo.co.in" class="auth_mail" title="E-mail the corresponding author ; Manvendra S. Kacholea
- 关键词：Host plant resistance ; Bifunctional α-amylase/trypsin inhibitor ; Pigeonpea ; H. armigera ; Reverse zymography ; Mass spectrometry
- 刊名：Pesticide Biochemistry and Physiology
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：125
- 期：Complete
- 页码：17-25
- 全文大小：1230 K
文摘
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This paper evaluates amylase inhibitor mediated defense of pigeonpea against H. armigera.
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Bifunctional amylase/trypsin inhibitor is purified from pigeonpea cultivar BSMR-736.
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It was found to be multi-isomeric glycoprotein shared homology with cereal type AIs.
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Upon ingestion it negatively affected growth rate of H. armigera.
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In-vivo studies indicated increase in midgut amylase activity of H. armigera.