Dynamic metabolic flux analysis using B-splines to study the effects of temperature shift on CHO cell metabolism
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- 作者：Veró ; nica S. Martí ; nez1 ; Maria Buchsteiner1 ; Peter Gray ; Lars K. Nielsen ; lars.nielsen@uq.edu.au" class="auth_mail" title="E-mail the corresponding author ; Lake-Ee Quek
- 关键词：B-DMFA ; B-spline fitting dynamic metabolic flux analysis ; CHO ; Chinese hamster ovary ; DMFA ; dynamic metabolic flux analysis ; L-DMFA ; linear dynamic metabolic flux analysis ; MFA ; metabolic flux analysis ; SSR ; variance-weighted sum of squared residuals
- 刊名：Metabolic Engineering Communications
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：2
- 期：Complete
- 页码：46-57
- 全文大小：2551 K
文摘
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New dynamic MFA framework using B-spline (B-DMFA) generates smooth fit.
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B-DMFA performs better than linear DMFA when fitting fast dynamic changes.
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Heuristic algorithm for knot placement dramatically reduced computation time.
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Temperature shifted cultures maintain a constant IgG volume specific productivity.
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CHO cells shifted to 32 °C have a 42% higher IgG titer due to larger cell volume.