Paraffin processing of stented arteries using a postfixation dissolution of metallic and polymeric stents

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• 作者：Ilia Fishbein^a ; ^b ; ^{fishbein@email.chop.edu" class="auth_mail" title="E-mail the corresponding author} ; Tre Welch^c ; David T. Guerrero^a ; Ivan S. Alferiev^a ; ^b ; Richard F. Adamo^a ; Michael Chorny^a ; ^b ; Rohit K. Gupte^a ; Yanqing Tang^a ; Robert J. Levy^a ; ^b
• 关键词：Stents ; Arterial tissue ; Histological processing ; Paraffin embedding ; Immunohistochemistry
• 刊名：Cardiovascular Pathology
• 出版年：2016
• 出版时间：November-December 2016
• 年：2016
• 卷：25
• 期：6
• 页码：483-488
• 全文大小：1716 K

文摘

Studying the morphology of the arterial response to endovascular stent implantation requires embedding the explanted stented artery in rigid materials such as poly(methyl methacrylate) to enable sectioning through both the in situ stent and the arterial wall, thus maintaining the proper anatomic relationships. This is a laborious, time-consuming process. Moreover, the technical quality of stained plastic sections is typically suboptimal and, in some cases, precludes immunohistochemical analysis. Here we describe a novel technique for dissolution of metallic and plastic stents that is compatible with subsequent embedding of “destented” arteries in paraffin, fine sectioning, major staining protocols, and immunohistochemistry.