Biodegradation of toxic chemicals by Pleurotus eryngii in submerged fermentation and solid-state fermentation

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• 作者：Bea-Ven Chang ; ^{bvchang@scu.edu.tw" class="auth_mail" title="E-mail the corresponding author} ; Yi-Ming Chang
• 关键词：Biodegradation ; Pleurotus eryngii ; Solid-state fermentation ; Spent mushroom compost ; Submerged fermentation ; Toxic chemicals
• 刊名：Journal of Microbiology, Immunology and Infection
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：49
• 期：2
• 页码：175-181
• 全文大小：407 K

文摘

The toxic chemicals bisphenol A (BPA), bisphenol F (BPF), nonylphenol (NP), and tetrabromobisphenol A (TBBPA) are endocrine-disrupting chemicals that have consequently drawn much concern regarding their effect on the environment. The objectives of this study were to investigate the degradation of BPA, BPF, NP, and TBBPA by enzymes from Pleurotus eryngii in submerged fermentation (SmF) and solid-state fermentation (SSF), and also to assess the removal of toxic chemicals in spent mushroom compost (SMC).

Methods

BPA and BPF were analyzed by high-performance liquid chromatography; NP and TBBPA were analyzed by gas chromatography.

Results

NP degradation was enhanced by adding CuSO₄ (1 mM), MnSO₄ (0.5 mM), gallic acid (1 mM), tartaric acid (20 mM), citric acid (20 mM), guaiacol (1 mM), or 2,2′-azino-bis- (3-ethylbenzothiazoline-6-sulfonic acid; 1 mM), with the last yielding a higher NP degradation rate than the other additives from SmF. The optimal conditions for enzyme activity from SSF were a sawdust/wheat bran ratio of 1:4 and a moisture content of 5 mL/g. The enzyme activities were higher with sawdust/wheat bran than with sawdust/rice bran. The optimal conditions for the extraction of enzyme from SMC required using sodium acetate buffer (pH 5.0, solid/solution ratio 1:5), and extraction over 3 hours.

Conclusion

The removal rates of toxic chemicals by P. eryngii, in descending order of magnitude, were SSF > SmF > SMC. The removal rates were BPF > BPA > NP > TBBPA.