Graphene oxide: A substrate for optimizing preparations of frozen-hydrated samples

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• 作者：Radosav S. Pantelic ; Jannik C. Meyer ; Ute Kaiser ; Wolfgang Baumeister ; Jü ; rgen M. Plitzko
• 关键词：Graphene ; Graphene oxide ; Cryo-EM ; Electron microscopy
• 刊名：Journal of Structural Biology
• 出版年：2010
• 出版时间：April 2010
• 年：2010
• 卷：170
• 期：1
• 页码：152-156
• 全文大小：679 K

文摘

Graphene oxide is a hydrophilic derivative of graphene to which biological macromolecules readily attach, with properties superior to those of amorphous carbon films commonly used in electron microscopy. The single-layered crystalline lattice of carbon is highly electron transparent, and exhibits conductivity higher than amorphous carbon. Hence, graphene oxide is a particularly promising substrate for the examination of biological materials by electron microscopy. In this manuscript we compare graphene oxide films to commonly used amorphous carbon films, describing the use of graphene in optimizing the preparation of unstained, vitrified biological macromolecules.