Euphorbia factor L2 induces apoptosis in A549 cells through the mitochondrial pathway

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• 作者：Minting Lin^a ; Sili Tang^a ; Chao Zhang^a ; Hubiao Chen^b ; Wenjing Huang^a ; Yun Liu^a ; Jianye Zhang^a ; ^{jianyez@163.com}
• 关键词：Euphorbia Factor L2 ; Caper euphorbia seed ; Euphorbia lathyris L. ; Anticancer agent ; Apoptosis ; Mitochondrial pathway
• 刊名：Acta Pharmaceutica Sinica B
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：7
• 期：1
• 页码：59-64
• 全文大小：1173 K
• 卷排序：7

文摘

Euphorbia factor L2, a lathyrane diterpenoid isolated from caper euphorbia seed (the seeds of Euphorbia lathyris L.), has been traditionally applied to treat cancer. This article focuses on the cytotoxic activity of Euphorbia factor L2 against lung carcinoma A549 cells and the mechanism by which apoptosis is induced. We analyzed the cytotoxicity and related mechanism of Euphorbia factor L2 with an MTT assay, an annexin V-FITC/PI test, a colorimetric assay, and immunoblotting. Euphorbia factor L2 showed potent cytotoxicity to A549 cells. Euphorbia factor L2 led to an increase in reactive oxygen species (ROS) generation, a loss of mitochondrial electrochemical potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase, suggesting that Euphorbia factor L2 induced apoptosis through a mitochondrial pathway. The cytotoxic activity of Euphorbia factor L2 in A549 cells and the related mechanisms of apoptotic induction provide support for the further investigation of caper euphorbia seeds.