Label free ultrasensitive optical sensor decorated with polyaniline nanofibers: Characterization and immunosensing application

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• 作者：Sutapa Chandra^aAuthor Vitae ; Reshma Bharadwaj^bAuthor Vitae ; Soumyo Mukherji^a ; ^b ; ^{mukherji@iitb.ac.in} ; ^{soumyo.mukherji@gmail.com}Author Vitae
• 关键词：Polyaniline nanofibers ; U-bent fiber optic sensor ; Immunosensing ; Spectroscopy
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：240
• 期：Complete
• 页码：443-450
• 全文大小：2926 K
• 卷排序：240

文摘

In situ synthesis of polyaniline (PAni) was done on uncladed optical fiber probe. Antibodies were immobilized on PAni modified fiber to develop an immunosensor. Conformational changes of polyaniline due to binding of protein molecules alter the optical evanescent wave absorbance characteristics of PAni. Initial studies show that this immunosensor can detect as low as 5 ng/ml (37 pM) of immunoglobulin.