An acid precipitation technique: A strip assay for the large-scale DNA polymerase activity screening
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- 作者：Maria Yu Kordyukovaa ; Valeriya N. Antipovab ; Vadim V. Rogachevskyc ; Nadezhda V. Zyrinab ; zyrina.nv@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：DNA polymerase activity screening ; GF/C glass fiber ; Bst DNA polymerase ; Large fragment purification
- 刊名：Analytical Biochemistry
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：513
- 期：Complete
- 页码：39-42
- 全文大小：645 K
文摘
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One GF/C strip, many radioactive samples on teeth.
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Low-cost and high-productive screening for DNA polymerase activity.
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The strip assay was validated by comparison with the glass fiber disk technique.
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The disposal of radioactive waste is facilitated.
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A new protocol for Bst DNA polymerase LF purification.