Plasmodium vivax apicoplast genome: A comparative analysis of major genes from Indian field isolates

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• 作者：Vishal Saxena^a ; ^{vishalsaxena@bits-pilani.ac.in} ; ^{vishalsaxena12@gmail.com} ; Shilpi Garg^a ; Jyotsna Tripathi^a ; Sonal Sharma^a ; Deepak Pakalapati^a ; Amit K. Subudhi^a ; P.A. Boopathi^a ; Gagandeep S. Saggu^a ; Sanjay K. Kochar^b ; Dhanpat K. Kochar^c ; Ashis Das^a
• 关键词：Plasmodium vivax ; Apicoplast ; Plastid DNA ; Codon usage ; Phylogeny
• 刊名：Acta Tropica
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：122
• 期：1
• 页码：138-149
• 全文大小：1860 K

文摘

The apicomplexan parasite Plasmodium vivax is responsible for causing more than 70 % of human malaria cases in Central and South America, Southeastern Asia and the Indian subcontinent. The rising severity of the disease and the increasing incidences of resistance shown by this parasite towards usual therapeutic regimens have necessitated investigation of putative novel drug targets to combat this disease. The apicoplast, an organelle of procaryotic origin, and its circular genome carrying genes of possible functional importance, are being looked upon as potential drug targets. The genes on this circular genome are believed to be highly conserved among all Plasmodium species. Till date, the plastid genome of P. falciparum, P. berghei and P. chabaudi have been detailed while partial sequences of some genes from other parasites including P. vivax have been studied for identifying evolutionary positions of these parasites. The functional aspects and significance of most of these genes are still hypothetical. In one of our previous reports, we have detailed the complete sequence, as well as structural and functional characteristics of the Elongation factor encoding tufA gene from the plastid genome of P. vivax. We present here the sequences of large and small subunit rRNA (lsu and ssu rRNA) genes, sufB (ORF470) gene, RNA polymerase (rpo B, C) subunit genes and clpC (casienolytic protease) gene from the plastid genome of P. vivax. A comparative analysis of these genes between P. vivax and P. falciparum reveals approximately 5-16 % differences. A codon usage analysis of major plastid genes has shown a high frequency of codons rich in A/T at any or all of the three positions in all the species. TTA, AAT, AAA, TAT, and ATA are the major preferred codons. The sequences, functional domains and structural analysis of respective proteins do not show any variations in the active sites. A comparative analysis of these Indian P. vivax plastid genome encoded genes has also been done to understand the evolutionary position of the Indian parasite in comparison to other Plasmodium species.