An RNA polymerase I-driven human respiratory syncytial virus minigenome as a tool for quantifying virus titers and screening antiviral drug

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• 作者：Yuan-Hui Fu¹ ; Ya-Ru Liu¹ ; Yan-Peng Zheng ; Nan Jiang ; Yue-Ying-Jiao ; Wei Li ; Xiang-Lei Peng ; Jin-Sheng He ; ^{jshhe@bjtu.edu.cn}
• 关键词：Human respiratory syncytial virus ; Gaussia luciferase ; Minigenome ; Antiviral drug ; Drug-screening
• 刊名：Chinese Chemical Letters
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：28
• 期：1
• 页码：131-135
• 全文大小：1098 K
• 卷排序：28

文摘

Human respiratory syncytial virus (RSV) is an important pediatric pathogen of lower respiratory tract worldwide. No vaccines and antiviral drugs are available. Herein the use of an RNA polymerase I-driven RSV minigenome for analyzing RSV replication and screening anti-RSV drugs was investigated. The RNA polymerase I (Pol I) was used to transcribe RSV minigenome from the constructed plasmid, designated pHM-RSV-Gluc, of minigenome cDNA which comprised trailer region, gene start sequence (GS), reverse complementary copy of Gaussia luciferase (Gluc) gene, gene end sequence (GE), and leader region in the direction of 5′–3′ end and was flanked by promoter and terminator of Pol I. The expression of Gluc was confirmed in pHM-RSV-Gluc transfected HEp-2 cells following RSV infection and had the characteristics of dose-dependent, which provided a rapid, sensitive, and quantitative method for quantifying virus titers and screening antiviral drugs.