Simultaneous quantitation of polymyxin B1, polymyxin B2 and polymyxin B1-1 in human plasma and treated human urine using solid phase extraction and liquid chromatography-tandem mass spectrometry
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- 作者：Min Menga ; min.meng@covance.com" class="auth_mail" title="E-mail the corresponding author ; Laixin Wanga ; Sherry Liua ; Othman M. Jabera ; Lihong Gaob ; Lynn Chevretteb ; Scott Reuschela
- 关键词：Polymyxin B1 ; Polymyxin B2 ; Polymyxin B1-1 ; Human plasma and urine ; LC&ndash ; MS/MS ; Selected reaction monitoring (SRM)
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 February 2016
- 年：2016
- 卷：1012-1013
- 期：Complete
- 页码：23-36
- 全文大小：1168 K
文摘
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Polymyxins are antibiotics which have been used for the treatment of gram-negative bacterial infections.
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Two LC–MS/MS methods were developed to quantify three polymyxins (B1, B2 and B1-1) in human plasma and treated urine.
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This is the first report for the quantitation of polymyxin B1-1 in biological matrixes along with polymyxin B1 and B2.
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The LLOQ achieved in this method is the lowest LLOQ published to date.
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These methods have been used to support regulated clinic studies.