Diclofenac in Arabidopsis cells: Rapid formation of conjugates

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• 作者：Qiuguo Fu^a ; ^b ; ¹ ; ^{fuqiuguo@zju.edu.cn} ; ^{qiuguo.fu18@gmail.com} ; Qingfu Ye^b ; Jianbo Zhang^c ; Jaben Richards^a ; Dan Borchardt^d ; Jay Gan^a
• 关键词：PPCPs ; Diclofenac ; Plant metabolism ; Non-extractable residue ; Arabidopsis thaliana
• 刊名：Environmental Pollution
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：222
• 期：Complete
• 页码：383-392
• 全文大小：1331 K
• 卷排序：222

文摘

A tiered approach involving 14C trace technique and liquid chromatography-high resolution mass spectrometry (LC-HRMS) was used to obtain a comprehensive fate of diclofenac in Arabidopsis (i.e. phase I, phase II, and phase III). Target, suspect, and non-target analysis was multi-applied to identify the metabolites. Eleven phase I and phase II metabolites were identified in Arabidopsis cells and validated in seedlings. >40% non-extractable residue was observed in cells after 120-h incubation with diclofenac. Acyl-glutamatyl-diclofenac was newly found phyto-transformation product of diclofenac.